Relative response and relative amount calculation

In case of single-level calibration, the relative response factor (RRF) is evaluated using response and amount values from calibration samples. Depending on the RF definition in the global calibration settings, one of the following formulas applies:

With RF defined as Response per amount:

With RF defined as Amount per Response:

where
RRF	Relative response factor
RelResponse	Relative response
RelAmount	Relative amount

The amount and concentration are calculated according to the following formulas, using the response value from the sample measurement:

With RF defined as Response per amount:

With RF defined as Amount per Response:

Amount = RRF * RelResponse * Amount_ISTD

where
Amount	Compound amount
RelResponse	Relative response
RRF	Relative response factor
Amount_ISTD	Amount of internal standard

For multi-level calibration, the relative response factor is evaluated from the calibration curve.

For details on calculation of concentrations, see Concentration and mass%.