If the calibration data of a calibrated compound is to be used to quantify uncalibrated compounds, the calibrated compound is identified in the processing method (Calibration node, Compound Table tab: under Mode, select Reference). Calculations are the same as for calibrated compounds. If the reference compound is quantified by an ISTD method, the ISTD is used for the uncalibrated compound in the same way as for the reference compound.
A missing reference peak results in a zero amount of the uncalibrated compound.
Optionally, a correction factor (Ref. correction) can be entered to multiply the response of the peak before the amount is calculated from the response factor of the reference compound.