UV confirmation

UV reference spectra are acquired from a reference sample under well-defined chromatographic conditions. You can confirm the identity of a compound by comparing the current spectrum at the peak apex with the UV reference spectrum. The application calculates a match factor for the two spectra.

The algorithm for the comparison is identical to the one used for the UV impurity check (see Match factor calculation). Background correction is optional, it can be selected in the processing method.

For UV confirmation, the match factor must be greater than a given limit to be colored green in the injection results. You set the match factor limit in the processing method. The resulting UV confirmation match factor is shown in the injection results.

 

UV confirmation match factor in injection results