Not identified peaks can be quantified using timed groups, either with a fixed response factor or with the calibration data of one of the calibrated compounds. Quantitation using a fixed response factor or calibrated compound data is signal-specific. In the latter case, if the calibrated compound is quantified by an ISTD method, the ISTD is used for the not identified peaks in the same way as for the calibrated compound.
For more information on timed groups, see Definition of a timed group.