Response type and response factor

There are different settings that allow you to choose which values are plotted on the x and y axis of the calibration curve:

RF definition

The response factor (RF) is a measure of the extent to which the signal changes if a compound is detected. It is defined as the ratio of the response to the compound amount, or vice versa. In the general method settings under RF definition, you can switch between Response per amount (default) or Amount per response. If you change this setting, you swap the x and y axis of the calibration curve.

 

Different RF definitions, Response set as Area

RF calculation:

RF = Response/Amount

or

RF = Amount/Response

Type of response

The response itself can be defined as Area, Area%, Height, or Height%. You can choose the response type individually for each compound.

Relative responses with ISTD

If you use internal standards (ISTDs) in your sample, relative amounts and relative responses are shown in the calibration curve. The calculation depends on the RF definition.

RF calculation:

RF = (Response/ISTD Response) / (Amount/ISTD Amount)

or

RF = (Amount/ISTD Amount) / (Response/ISTD Response)

Log/log curve model

If you select the curve model log/log for a compound, the amount and response are both plotted as logarithmic values.

You can use the log/log model in combination with both RF definitions, with all response types, and with internal or external standards.

 

Example for log/log model with external or internal standards

RF calculation for the examples shown above:

RF = log(Response) / log(Amount)

RF = log(Response/ISTD Response) / log(Amount/ISTD Amount)

Scaled response

If you select a scaled response, the response is plotted as a calculated value while the amount is plotted without modification.

 

Examples for a scaled response using the sqrt function

RF calculation for the example shown above:

RF = sqrt(Response) / Amount

or

RF = Amount / sqrt(Response)