Add UV reference spectra

You can extract the UV reference spectra at the apex of your peaks. The UV reference spectra are added to the processing method. By comparing a reference spectrum with the current spectrum at the peak apex, you can confirm the identity of a compound.

Prerequisites

To be able to carry out the procedure as described, you need the privilege Processing Method > Edit identification parameters. Privileges are configured in the Control Panel.
You have linked your injections to a processing method that contains compound identification parameters.
If the project enforces method approval, the method status must be Generic. For details, see Method approval.

Set the confirmation parameters

In the Processing Method window, select the Compounds > Spectra node.
Select the UV Confirmation tab.
Enter the required values and options:
- UV confirmation match factor limit
  Match factor higher or lower than the given threshold value will be displayed with a green or red background in the injection results.
- Lower wavelength range [nm], Upper wavelength range [nm]
  Wavelength range to use for the comparison. By default, the whole range is used.
  The used wavelength range is also highlighted in the reference spectra in the Compounds > Spectra node.
- Subtract baseline spectra
  
  If checked, the spectra are background-corrected by subtracting the baseline spectra.

For all identified compounds

Right-click anywhere in the Compound Table tab, then select Auto Extract UV spectra.

The spectrum icon is shown in the UV Reference Spectrum column for all compounds for which a spectrum has been extracted.

For a specific compound

In the chromatogram of the relevant injection, right-click the peak of the specific compound.
From the context menu, select Add reference spectrum to method.
In the Add reference spectrum to method dialog, select and the correct compound name.

The spectrum icon is shown in the UV Reference Spectrum column for the selected compounds only.

Delete a UV reference spectrum

In the compound table of the Spectra node, right-click the compound, then select Delete selected reference spectrum.

Display a UV reference spectrum

In the compound table of the Spectra node, click .

In the UV Spectrum window, click to show the reference spectrum for the selected compound.

The legend title of the reference spectrum contains a Ref tag and the expected retention time of the compound for which the spectrum has been extracted.