During reprocessing of a sequence or single run, the application runs all parts of the processing method. The application uses all currently provided parameters from injection list and method to update all results, including the calibration curve.
The main difference to requantification is that the calibration curve itself is not updated, but is used to recalculate the results. The following sections describe the differences in more detail.
With Reprocess All, all calibration curves are updated. The updated calibration curves are used to recalculate the results. Reprocess All is typically used if calibration parameters have been changed and the entire sequence must be recalculated.
With Reprocess Selected and selecting calibration standards, only the corresponding calibration curve is updated. The updated calibration curve is used to recalculate the results of selected samples. Reprocess Selected is typically used if integration parameters have been changed and shall be checked for a specific chromatogram. Another example is when you change a single injection and want to propagate this change to another specific injection (select both injections in this case).
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In case of bracketed calibrations, Reprocess Selected may lead to wrong results. Use Reprocess All or Requantify Selected for bracketed calibrations. |
All calibration curves remain unchanged. This action will execute only the quantitation (no identification or calibration of the injection). The existing calibration curves are used to recalculate the results.
When working with a result set, the calibration curves used to requantify are the individual calibration curves that are available for each injection in the result set. When working with single runs, the calibration curve that is stored in the linked method is first copied to the single run results. This calibration curve is then used to requantify.
Requantify Selected is typically used after a manual modification of an injection, especially if the sample is part of a sequence with a complex calibration (e.g. bracketed calibration). Requantify can also be used after manual operations to re-compute Custom Calculation results (in that case, all injections of the result set must be selected).
Manual modifications such as manual integration or manual identification will be propagated to the processing method during reprocessing and will influence the new method calibration curve. Consider requantification if you want to compute results with this calibration curve.
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Requantify |
Reprocess | |
|---|---|---|
Method modifications | ||
| No |
Yes |
|
Yes | Yes |
| When changed, any already computed amount and concentration are removed | Yes |
|
No | No |
| Only if all injections of a result set are requantified | Only if all injections of a result set are reprocessed |
Manual modifications | ||
|
Yes | Yes, except for cleared corrections (see Reprocess data) |
|
Yes | Yes |
Sample parameters (injection list) | ||
| Yes |
Yes |
|
No | Yes |
|
No | Yes if you reprocess all injections of a result set |