Set up a bracketed calibration
In the Injection List window, select the type of bracketing you want to use from the Bracketing mode drop-down list. The following bracketing modes are available:
| If you choose one of the predefined bracketing modes, the Run type column will become inactive. All calibration points from preceding brackets will be cleared automatically when a new bracket opens. |
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Ensure that:
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NoneNone
No bracketing. Samples and sets of calibration standards are processed in the order of acquisition. Amount and concentration of samples are computed with the calibration curve that is available at this point in time. Amount and concentration of calibration standards are computed with the calibration curve built from all standard injections present in the result set.
OverallOverall
The calibration curve is calculated with all calibration standards in the sequence, starting with the first one and finishing with the last one. All samples are reprocessed after the calibration curve has been calculated.
Non overlapNon overlap
You must have at least three groups of standards in your sequence, and at least two standards in the middle blocks. The standards from the group in the middle of the sequence are used in one calibration curve only.
If there are more than two standards in the middle block, they will be divided and allocated to the preceding and subsequent groups. With uneven numbers of standards in the middle block, the extra standard is allocated to the preceding group.
OverlapOverlap
You must have at least three groups of standards in your sequence. Standards from the group in the middle of the sequence are used in two calibration curves (with the preceding and with the following block).
CustomCustom
Create brackets as required. Under Run type, you can choose for each calibration standard individually which calibration levels shall be cleared. If you do not choose any run type, a bracket will be averaged with its predecessor.
In the example shown above:
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Samples 1 and 2 have a calibration curve with 4 calibration points.
Samples 3 and 4 have a calibration curve with 6 calibration points.
With Clear all calibration in lines 1 and 5, the above example would correspond to an Overlap bracketing.