After the peaks have been integrated and identified, the next step in the analysis is quantitation. Quantitation uses peak area or height to determine the amount of a compound in a sample.
A quantitative analysis involves many steps which are briefly summarized as follows:
Analyze the sample containing an unknown amount of the compound to obtain the response due to the unknown amount.
Compare the response of the unknown amount to the response of the known amount to determine how much of the compound is present.
To obtain a valid comparison for the unknown sample response to that of the known sample, the data must be acquired and processed under identical conditions.