Quantitation using a manual factor

The software allows you to quantify an identified compound that is based on a fixed response factor (Manual Factor column). In this case, the compound amount is calculated using the fixed response factor:

Amount = Response * Manual Factor

where
Manual Factor	Fixed response factor
Response	Response can be Area, Area%, Height, Height%, Rel. Area, or Rel. Amount (see Response type and response factor)

For details on calculation of concentrations, see Concentration and mass%.

Using a manual factor with an ISTD method

If the compound amount is quantified using the fixed response factor and ISTD, the formula is read as follows:

Relative area = Area / Area_ISTD

or:

Relative height = Height / Height_ISTD

The amount is then calculated as follows:

Amount = Relative Area * Manual Factor * Amount_ISTD

or:

Amount = Relative Height * Manual Factor * Amount_ISTD

For details on calculation of concentrations, see Concentration and mass%.

Dependency of manual factor and response factor (RF)

With RF defined as Response per amount (default setting):

RF = 1 / Manual Factor

With RF defined as Amount per response:

RF = Manual Factor

For more information on the response factor, see Response type and response factor.