You can choose to normalize amounts in the general calibration settings of a processing method.
The normalization analysis has the same disadvantage as the Area% and Height% calculations. Any changes that affect the total peak area will affect the concentration calculation of each individual peak. The normalization analysis should only be used if all components of interest are eluted and integrated. Excluding selected peaks from a normalization analysis will change the reported results in the sample.
For detailed information on timed groups, see Definition of a timed group.
If individual peaks are calculated in timed groups, these individual peaks are not included a second time in the total amount; they are already included in the timed group amount.
The default normalization factor is 100.00 to create Norm% results. However, you can set a different number and unit in the method. The total amount is the sum of all calculated compound and timed group amounts, independent of the signal of the compound main peak.
You can select whether ISTD compounds are included in the calculation. If excluded (default setting), the ISTD amount is not added to the total amount, and no compound normalization amounts are calculated for the ISTDs.
For named groups the group amount is not included in the total amount. However, if an ISTD has been explicitly added to this named group, it will add to the group amount, even if ISTD is excluded from normalization. This can result in normalization amounts greater than 100% for the named group.