If you use time reference compounds, the application corrects the absolute retention time windows based on possible shifts observed by specific reference compounds.
One or more compounds in the processing method can be marked as time reference compounds. For each compound or timed group, a time reference compound can be selected to correct the expected retention time. The extent of correction can be adjusted by an individual correction factor, which can be selected for each compound for correcting the expected retention time (column Factor, default = 1).
If a compound has a time reference compound assigned to it, the expected retention time will be corrected by the shift of the assigned time reference compound. The compound identification algorithm will use the corrected expected retention time for identifying the peak in the chromatogram. In case of timed groups, the time ranges are corrected by the shift. Generally the shift is corrected by the entered correction factor. If an associated reference compound is not found in the chromatogram, the linked peaks and time groups are not identified.
If you use time reference compounds and RRT reference compounds, both expected RTreference and expected RTcompound are adjusted, so that the RRT remains constant.
| When using time reference compounds, all compounds and timed groups must have a time reference compound assigned to them. Otherwise the method is inconsistent and cannot be used for reprocessing. |