There is one global number of calibration levels per processing method for all compounds. The number of calibration levels defines how many points (amount, response) are used to calculate the calibration curve. You define each level by processing the corresponding calibration standard. For each compound, the calibration curve shows the calibration points that have been used to calculate the averages.
When re-running a calibration standard, or when processing further calibration standards of a given calibration level, the calibration point for that level is updated. If you chose to use average values in the Curve calculation settings, the calibration point is updated by the average value of the new measured point and all already existing value(s) (see Modes of using individual points),
The collection of calibration points can be controlled by the Run Type as follows:
No selection: A new point will be added to the calibration curve.
Clear all Calibration: All calibration points for all calibration levels are deleted before the new calibration points are saved.
Clear Calibration at Level: All calibration points for the given calibration level are deleted before the new calibration points are saved.
Reprocessing the same calibration standard injection multiple times will update the same calibration point in the curve and not add new points.