If multiple peaks are within the retention time window, there are different ways how to identify a particular peak. You can choose between the following values for Peak match in the compound identification parameters:
First: Use the first peak in the retention time window.
Last: Use the last peak in the retention time window.
Closest (default setting): Use the peak that is closest to the expected retention time.
Closest nested: When there is a base peak and a nested peak, and both have the same retention time, use the nested peak.
Largest area: Use the peak with the largest area in the retention time window.
Largest height: Use the peak with the largest height in the retention time window.
If the conflict cannot be resolved, none of the peaks will be identified and a warning will be written into the processing log.
If you installed the 2D-LC Add-on: In the second dimension, the distance to the closest peak is calculated differently. See 2D-LC Analysis Add-on Software help.
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