Imported EZChrom method par... (Data Analysis)
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Imported EZChrom method parameters

The following parameters of the EZChrom method are loaded into a new processing method:

  • Compound name

    Compound names can be redundant if used with different signals.

  • Signal name

  • Expected retention time

  • Retention time window:

    Iin OpenLab CDS, the absolute RT Window corresponds to half the EZchrom value. The Retention time window value is therefore divided by two.

  • Integrations events (if the method has been created with EZChrom A.04.05 or higher):

    • All the integration events defined in the EZChrom method with their associated times, except Force BL Start and Force BL Stop that do not exist in OpenLab CDS.

    • For the following integration events, no stop times are imported as they are not used in OpenLab CDS: Width, Threshold, Shoulder sensitivity, Minimum area, Reset baseline, and Reset baseline at valley.

    • If several signals exist in the EZChrom method, each with its own integration events table, the same number of signals are created in the imported method with the corresponding integration events table.

      Signal names can be different in EZChrom and in OpenLab CDS. To use the imported EZChrom integration events in a method using different signal names, copy the EZChrom integration events to the corresponding signal (see Copy integration events to another signal).

In addition, the following calibration parameters are imported:

EZChrom parameter

OpenLab CDS parameter

Comment

Last calibration date

Just after the import, the Last calibration date field displayed in the method shows the date and time of the import. After processing the samples, this is the date of the processing.

Disabled calibration points

Units

Concentration unit

Amount unit

Quantitate

Response

Area or hight

Fit type

Possible values:

  • Point to point

  • Linear

  • Quadratic

  • Cubic

  • Average RF

Mode = Curve

Curve model:

  • Linear

  • Linear

  • Quadratic

  • Linear

  • Average RF

The curve models Point to point and Cubic are not supported in Data Analysis. If you import a method using these parameters, the curve model is set to Linear.

The curve model is set to Log/log if the Scale factor in EZChrom is log(Amt) & log (Resp).

Zero

  • Selected

  • Clear

Origin

  • Forced

  • Ignore

Origin is set to Forced for the curve model Average RF.

Scale

Response scaling

In the following cases the response scaling is set to None :

  • The scale factor is not supported in OpenLab CDS

  • The method uses internal standards

  • Curve calculation is set to From average per level

  • for timed groups

If the Scale in EZChrom is log(Amt) & log (Resp), Response Scaling in OpenLab CDS is set to None, and the curve model is set to Log/log.

Weighting method

Weighting method

Levels

Levels

STD ID

Mode = Reference curve

Curve reference = Compound with the ID specified in the STD ID column in EZChrom

Manual RF

Mode = Manual factor

Manual factor = 1/Value of Manual factor in Ezchrom

ISTD ID

not a separate value

Internal standard is set in the general calibration parameters.

Allow level specific ISTD amounts is selected.

For ISTD compounds in EZChrom, Is ISTD is selected in OpenLab CDS. Associated ISTD is the compound with the ID specified in the ISTD ID column in EZChrom.

RT Update (in EZChrom, this is available only for compounds)

  • None

  • Run

  • Calib

  • Run and calib

RT Update

  • never

  • after each run

  • after calibration standards

  • never

The option Run and calib is not supported in Data Analysis. If a method is imported using this option for a compound, it will be set to the default value Never in Data Analysis.

Automatically average

  • Selected

    Optional: Rolling average with n replicates

  • Cleared

Curve calculation

  • From average per level

    If a rolling average was configured: the average of the last n replicates is imported.

  • Individual points

Note that the Concentration calculation is set to Amount*Multipliers/Dil. factor in Data Analysis.

Group type

  • Named peak

  • Uncalibrated range

  • Calibrated range

Type

  • Icon for named groups

  • Icon for timed groups

  • Icon for timed group

Named peaks group

Named group

Compounds that are part of the named peak groups are also selected in the named group in Data Analysis.

Uncalibrated range group

Timed group, with Quantify each peak individually selected and Include identified peaks cleared

For an uncalibrated range group, Mode is automatically set to Manual factor, and Expected RT is set to 0.

If no value is set in EZchrom for the Manual RF, Manual factor is set to 1 in Data Analysis.

Calibrated range group

  • Include named peaks (selected or cleared)

  • Calculate concentrations for unnamed peaks in group (selected or cleared)

Timed group

  • Quantify each peak individually (selected or cleared)

  • Include identified peaks (selected or cleared)

Calibration Curve: Amount Ratio

Calibration curve: Relative amount

Calibration Curve: Height Ratio

Calibration curve: Relative response

Calibration Curve: Residual

Calibration curve: Residual per level

For numeric values that are defined with a precision > 9 digits in EZchrom, the imported values are rounded to 9 digits in Data Analysis.

If a value from Ezchrom is outside of an acceptable range for Data Analysis, it is set to its default value.

Log file

After the import, you find a log file next to the imported method. The log file lists all parameters that could not be imported.

Calibration Curve

If you import an EZChrom method that contains a calibration curve, the amounts and responses of the corresponding calibration standards are imported as well. The calibration curve is then built using the imported points and the imported calibration settings. This allows you to continue using your calibration curve.

See Also