Following an autotune, you should examine the tune report:
Tune Timestamp: the date and time the instrument was tuned.
Save Timestamp: the date and time the tune file was saved.
The mass assignments shown in the upper "profile" part of the display should be within +/- 0.2 m/z of the specified ions.
The peak widths (PW) of these three peaks should be 0.6 +/- 0.1 m/z.
The mass assignments shown in the lower "scan" part of the display should be within +/- 0.1 m/z of the specified ions.
The relative abundances should show that the peak at 69 m/z is the largest. Relative to that peak, the one at 219 m/z and the one at 502 m/z should be in the range specified for the autotune you performed.
The isotope (Iso) mass assignments should each be 1 m/z greater than the mass assignments of the parent peaks.
The isotope (Iso) ratio figures (indicating the relative abundances of the naturally occurring isotopes) should be close to the theoretical values of 1.08 for m/z 69, 4.32 for m/z 219, and 10.09 for m/z 502.
If mass 28 is greater than mass 18, there may be an air leak somewhere in the system. Exceptions are when it is within 1 hour of venting, or during the first autotune after refilling the calibration vial. See the Troubleshooting section of the online help for information about how to isolate air leaks.
When running an advanced autotune, the autotune steps selected to run will be shown on page 2 of the report.