If no peaks appear in your analysis but the MSD passes tune, the problem is probably related to your GC. Possible problems include:
Check the sample dilution. If the sample was diluted incorrectly, the concentration may be too low to allow detection of the analytes.
The target analytes may simply not be present. If the analyzer turned off for the solvent peak, then no peaks may be detected.
In a system equipped with an ALS, if the syringe is missing or not installed correctly, the ALS may continue to function but no sample will be injected so no peaks will be seen. Check the ALS. Make sure a syringe is correctly installed.
If the vial is empty, or almost empty, the syringe may not pick up any sample. Check the sample vial and make sure it is not empty.
If the septum is not sealing, the sample can be forced out the septum. Check and, if necessary, replace the septum. See the user information supplied with your GC.
If the column nut is loose, the sample can leak out. If necessary, tighten the nut slightly.
CAUTION! Over tightening the nut can crush the column.
If you accidentally make a split mode injection when the method expects a splitless injection most of the sample will be lost. Make sure you use the correct injection method.