Enables you to change one or more of the signal settings at the specified time.
Select the signal to change from the drop-down list.
The wavelength at which the absorbance of the sample is measured as a function of time. The absorbance at the reference wavelength is subtracted from the absorbance at the sample wavelength.
191 to 949 nm in steps of 1 nm
The bandwidth of the sample wavelength. The bandwidth determines the wavelength range over which the absorbance is measured. For example, a sample wavelength of 254 nm with a sample bandwidth of 20 nm measures absorbance from 244 to 264 nm.
1 to 400 nm (depending on the sample wavelength) in steps of 1 nm
The wavelength at which a reference absorbance is measured as a function of time. Mark the check box to switch on the reference wavelength. The reference wavelength compensates for fluctuations caused by changes in baseline absorbance, for example due to changes in solvent composition during gradient elution.
191 to 949 nm or Off (no reference)
The bandwidth of the reference wavelength.
1 to 400 nm in steps of 1 nm, but dependent on the reference wavelength. Mark the check box to switch on the reference bandwidth. For reference wavelengths less than 390 nm or greater than 750 nm, the bandwidth is restricted to twice the difference between the reference wavelength limit and the reference wavelength value.