This parameter defines the volume of mobile phase displaced by one stroke of piston 1. The stroke volume of the pump influences the mixing performance and gradient linearity. When the stroke is set to AUTO (default), the pump selects a stroke volume based on the set flow rate.
The pump uses 20 μl strokes up to a flow of 20 μl /min and increases the stroke in a linear fashion up to 100 μl stokes at 100 μl /min flow rates.
You can set the minimum stroke between 20 and 100 μl.
If you select a minimum stroke that is smaller than the one that would be used by the pump operating in AUTO mode, the pump will use the higher stroke volume.