The Sample Flush Volume is the additional volume of sample loading solvent that you want to use to flush the analyte onto the enrichment column. The Sample Flush Volume depends on the Injection Path Volume; for example, if the Injection Path Volume is 1.6 µl and you want to flush this volume with 2.5 times its volume of solvent, set the Sample Flush Volume to 4 µl.
Enter the sample flush volume in the Volume field.