The luminescence that you measure with the fluorescence detector has different characteristics depending on the chemistry of your application. The characteristics of your sample determines whether you need to use fluorescence detection mode or phosphorescence detection mode. For more details, see the Agilent Fluorescence Detector Reference Manual.
Choose the Fluorescence Mode option to measure luminescence from samples that emit fluorescence.
Choose the Phosphorescence Mode option to measure luminescence from samples that emit phosphorescence. When you choose to switch on the Phosphorescence detection mode, the two parameters Delay and Gate are activated.
Delay |
Sets a waiting period, during which the lamp flashes, before the FLD starts to measure. Limits: 0 to 5000.0 μs in steps of 0.1 μs. |
Gate | Sets a measurement time period after the lamp has flashed. Limits: 20.0 to 5000.0 μs in steps of 0.1 μs. |