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Fluorescence Scan Range is available only if the application supports it. It is not available with the G1321C or G7121A FLDs. |
The Fluorescence Scan Range panel enables you to set up a fluorescence scan. The fluorescence scan can be used to collect emission and excitation spectral information in a single task. It is most suitable for the characterization of single compounds, and for checking the purity of eluents. The contents of the flow cell must be kept constant during the measurement; use off-line measurements with the FLD cuvette or stopped-flow conditions in chromatography.
Enter the wavelength ranges for excitation and emission. The Step size determines the number of data points per spectrum. The time per scan (in seconds) is calculated automatically and displayed.
From ..to | These two fields define the lower and upper limits of the excitation scan. Limits: 200 to 1200 nm |
Step | The size of the wavelength increments. Limits: 1 to 20 nm. |
From ..to | These two fields define the lower and upper limits of the emission scan. Limits: 200 to 1200 nm |
Step | The size of the wavelength increments. Limits: 1 to 20 nm. |