To ensure that the peak of interest detected by the detector is collected in the fraction collector, a delay volume (in µL) must be specified for each peak detector used. You can enter a calculated volume or a volume measured by a Delay Volume Calibration procedure (use the Agilent Lab Advisor application).
|
The run time for a system with a fraction collector must be extended by the delay time due to the delay volume, to ensure the complete collection of all compounds. The run time is calculated as: Run time = End of last peak + delay time where delay time = delay volume/flow rate |
|
If multiple detectors are used, to prevent damaging detectors with low resistance to an increase in back-pressure (for example, RID and FLD) caused by a blockage in the fraction collector, the ones with the highest pressure-resistance should be placed first, and the ones with the lowest pressure-resistance should be placed last. |