This parameter defines the volume of mobile phase displaced by one stroke of piston 1. The stroke volume of the pump influences the mixing performance and gradient linearity. When the stroke is set to Automatic (default), the pump selects a stroke volume based on the set flow rate.
The stroke of each channel is based on the flow rate per channel, not on the total flow. The pump uses 20 µL strokes up to a flow of 20 µL /min and increases the stroke in a linear fashion up to 100 µL stokes at 100 µL /min flow rates.
You can set the minimum stroke between 20 and 100 µL (G1311A/B/C, G5611A, G5654A and G7111A/B) or 5 and 100 µL (G4204A, G7104A/C and G7131A/C).
If you select a minimum stroke that is smaller than the one that would be used by the pump operating in Automatic mode, the pump will use the higher stroke volume.