Gradient Shift

When the chromatographic separation in the first dimension requires a gradient elution, it may be necessary or desirable to change the solvent gradient in the second dimension in line with the changing solvent composition of the first dimension. This is handled by a gradient shift, which is set up in the second dimension gradient timetable.

In the upper case, the second dimension solvent gradient does not change, despite the changing composition of the solvent in the first dimension.

In the lower case, the gradient composition in the second dimension is shifted to match the changing solvent composition in the first dimension.