These parameters control the back-pressure regulator (BPR) of the SFC Control Module.
BPR Pressure |
The BPR controls the pressure at the outlet of the system. This represents the pressure experienced by the detector and its flow cell, and the pressure seen at the outlet of the column. You can set a BPR Pressure between 100 and 400 bar. BPR Pressure has a significant affect on chromatographic separation and detector noise. Optimal settings are in the range of 140 - 250 bar. |
BPR Temperature | As the mobile phase passes through the BPR, it expands as pressure reduces. This expansion is endothermic, and causes cooling of the BPR which is offset by heating the BPR. The BPR Temperature should be set above ambient; a nominal value of 60 °C is appropriate for most all applications. The BPR Temperature has little effect on chromatography. |