The Draw Speed determines the rate at which the plunger draws sample from the vial. Set the speed to an appropriate value for your sample. For viscous samples, use a slow Draw Speed.
|
Type | Syringe (μL) | Min (μL/min) |
Max (μL/min) |
|---|---|---|---|
Wellplate sampler | 100 | 10 |
1000 |
|
Wellplate sampler |
900 | 10 | 1000 |
| μ Wellplate sampler |
8 | 0.7 | 20 |
| μ Wellplate sampler |
40 | 0.7 | 250 |
The Eject Speed determines the rate at which the plunger ejects sample from the metering device. If you are injecting large volumes of sample, setting a high Eject Speed will shorten the time needed for an injection cycle. For viscous samples, use a slow Eject Speed.
See Draw Speed above
|
To achieve the maximum speed, preslit septa are recommended. |
The Draw Position is a vertical offset that enables you to position the needle a specific distance (in mm) away from its standard position. The Draw Position offset is calculated from the bottom of the well, with a slight amount of clearance to cover tolerances. Use a negative offset to touch the well bottom with the needle. Use a positive offset to raise the needle higher in the well to clear precipitates on the bottom of the well, or to select one phase of a biphasic sample.
|
The default position (Draw Position = 0.0) is a vertical offset of +1.0 mm. |
|
Wellplate sampler |
-10 to 50 mm relative to the standard position |
|
μ Wellplate sampler |
-10 to 50 mm relative to the standard position |
This ensures that the temporary vacuum, which originates from the drawing of liquid from the sample vial, dissipates. The needle stays first on the seat for the equilibration time, then after drawing sample from the vial, remains there for the specified equilibration time.
Limits: 0.0 to 100.0 seconds (default is 2.0 seconds).
The Sample Flush-Out Factor ensures that the sample is thoroughly flushed out of the syringe and past the capillary seat and valve. The factor is part of the flush-out volume formula, which is calculated by the autosampler firmware.
The volume is calculated as:
factor x (injection volume + seat capillary + valve volume).
The Sample Flush-Out Factor is preset to 5.0 at the factory.
The preset Sample Flush-Out Factor is correct for most methods. However, for unusually viscous samples, you should increase the Sample Flush-Out Factor to obtain the desired degree of flushing to prevent sample carry-over.
Limits:
From 0.1 to 20.0 (default is 5.0).
The Vial/Well Bottom Sensing feature allows the needle to detect non-uniform well bottoms and adjusts the depth of the needle position to 1 mm above the detected bottom of the vial or well.
You can use Vial/Well Bottom Sensing in combination with the Draw Position to customize the draw position of the needle.
You may want to turn off Vial/Well Bottom Sensing to increase speed of injection, or to avoid the needle touching the bottom of the well if a sample precipitate could clog it.
To turn on Vial/Well Bottom Sensing, click the check box. The default setting is off.