Options (Multisamplers)

The contents of this tab are set automatically during autoconfiguration. For offline usage, you can set the parameters manually.

Metering

Shows the currently installed metering device. For offline usage, click the down arrow to select a different metering device.

Left Needle installed

This check box is marked to indicate that your system is equipped with a dual-needle option. When the check box is marked, the Left Needle section is enabled.

Left Needle installed is not supported for G5668A or G7137A.

Left Needle

This section is enabled only when the Left Needle installed check box is marked.

Loop	Shows the currently installed loop capillary. For offline usage, click the down arrow to select a different loop capillary.
Seat	Shows the currently installed seat capillary. For offline usage, click the down arrow to select a different seat capillary.

If the Left Needle parameters are equivalent to the Right Needle parameters, then Alternating Needle Usage, which increases sampling efficiency, is possible.

Right Needle

Loop

Shows the currently installed loop capillary. For offline usage, click the down arrow to select a different loop capillary.

Seat

Shows the currently installed seat capillary. For offline usage, click the down arrow to select a different seat capillary. If a multi-draw seat capillary is selected, then multi-draw is enabled.

The needle seat capillary volume is used for the Automatic Delay Volume Reduction option and ISET.

If a Multi-draw Seat Capillary is selected, multiple injections can be made to the extended capillary volume, and the display shows Multi-draw enabled. Note, that, if a Multi-draw seat capillary is installed, the significantly higher volume of this extension capillary is used instead of the needle seat volume to calculate the maximum injection volume, which then no longer needs to be specified.

• If no Multi-draw seat capillary is installed, the maximum injection volume is determined by the smallest volume (metering head or loop capillary) currently installed:

  • if metering head volume < loop capillary volume:

    Max. injection volume = metering head volume

  • if metering head volume > loop capillary volume:

    Max. injection volume = loop capillary volume

• If a Multi-draw seat capillary is installed, it holds n × the injection volume of either the metering head or the loop capillary (whichever is smaller). Once the seat capillary is filled, one additional draw is made, so that the maximum injection volume is calculated as follows:

  • if metering head volume < loop capillary volume:

    Max. injection volume = (n+1) * metering head volume

  • if metering head volume > loop capillary volume

    Max. injection volume = (n+1) * loop capillary volume

For dual-needle operation, Multi-draw is disabled, even when a Multi-draw seat capillary is installed.

The Metering, Loop and Seat fields can be used to configure an offline instrument. When you switch to online mode, this information is read directly from the instrument. To change the configuration of an online instrument, use the Capillaries setup options from the Dashboard context menu.

Pressure Sensor installed

This check box is marked to show that a Pressure Sensor is installed.

Thermostat installed

This check box is marked to show that a sample cooling device or thermostat is installed.

• Mode

  Select Constant temperature mode to set the temperature using the Thermostat section of the Multisampler Control parameters. Use this mode to store samples at a constant temperature across multiple runs. This is the default mode, which is recommended in most cases.

  Select Variable temperature mode to set the temperature using the Thermostat section of the Advanced Method Setup parameters. In this mode, the temperature can be varied from run to run.

  Use Variable temperature mode with care. All samples in the storage compartment are exposed to the set temperature for any current method. Unintended change of the sample temperature may cause degradation. It may take some time to reach the set temperature.

• Thermostat

  Click the down-arrow and select the installed device (Cooler or Thermostat) from the drop-down list.

  The Multisampler Cooler does not offer a heating function. A temperature higher than the previous setting, or higher than ambient, may only be reached after a long time, if ever.

Multi-wash installed

This check box is marked to show that the multi-wash option is installed, and the Multi-wash option in the Needle Wash section of the Method parameters is available.

You cannot use multi-draw with the multi-wash option. If you use a multi-draw capillary in a multisampler with the multi-wash option, the multi-draw capability is ignored.

Vial Rack

Click the down-arrow and select the reference vial rack that is installed in your multisampler from the drop-down list.

Bypass capillary

Click the down arrow and select where the bypass capillary is installed (if any). When installed, the bypass capillary allows the configuration of a minimized injection path to reduce flush times and allow the dual-needle configuration to be used in single-path mode. In this case, the needle where the bypass is installed is no longer available for injection.

Define Sample Containers

Displays the Define and edit Wellplates dialog box, which contains a list of standard preconfigured wellplates plus any custom wellplates that have been added.