This section is available only for a dual-needle Multisampler with identical left and right flow paths (Seat capillary and Loop capillary), and Alternating Needle selected in the Injection section of the method setup.
Overlapped injection provides faster throughput of samples by allowing the preparation of the next injection while the current injection is being completed. The net effect is more samples performed during a given period of time, although the actual individual injection times remain constant. When Smart Overlap is employed, the next sample is taken up and injected into the Multisampler by the alternate needle a specified time after the first sample has been injected.
Enable Smart Overlap | Mark this check box to enable the Smart Overlap function. |
After Period of Time | Specify the time (in minutes) that the Multisampler waits after injection of a sample before taking up and injecting the next sample. |
The parameters for overlapped injection are specified as part of the method. There are no parameters for overlapped injection to be specified in the sequence/worklist table. However, there are important restrictions to using Overlapped Injection in sequences/worklists:
In the sequence/worklist, all injections where you wish to use overlapped injection must use the same method. This is because the method provides the necessary information for sample location and volume to trigger the events leading up to the next injection while the current injection is being completed. Where different methods are specified, the sequence/worklist continues, but the overlapped injection is ignored.
Control samples may NOT be used. If a control sample is inserted in the sequence/worklist, there will be no overlapping in the following injections because the Control sample might fail and the sequence/worklist would then be stopped.
Important restrictions for using Pretreatment/Injector Programs with Overlapped Injection:
Overlapped Injection can handle pre-injection tasks but not post-injection tasks. This is because the next sample is being prepared for injection during the current injection.
Some injector program commands, such as INJECT, may not be used with Overlapped Injection. For details, refer to the help for the specific command.