Dilution (Preparative Sampler)

The Dilution section is available only for the Analytical Operation Mode, and only when Enabled in the Sample Dilution section. It allows you to dilute concentrated samples before injection.

Ensure that you use a vessel with sufficient volume to hold the diluted sample.

The table shows the dilution workflow; the following parameters must be specified:

Draw Dilution Solvent

  • Dilution Factor

    Choose this option to specify a dilution factor in the adjacent field

  • Dilution Volume

    Choose this option to specify a volume of solvent with which to dilute the sample. Specify the volume in the adjacent field.

  • Dilution Solvent

    Click the down-arrow and select how to specify the location of the dilution solvent from the drop-down list. You can select from Location (see Specifying a Location), Current Container, Sample+ , Location+, Container and Drawer.

Draw Concentrated Sample

Specify the volume of concentrated sample to draw (in µL) in the Sample Volume field.

Eject to Dilution Position

    Specify the location for the diluted sample.

  • Required Volume

    This field shows the minimum required volume of the vessel.

  • Dilution Position

    Click the down-arrow and select how to specify the location of the dilution solvent from the drop-down list. You can select from Location (see Specifying a Location), Current Container, Sample+, Location+, Container and Drawer.

Use high recovery vials if the volume of the dilution is less than the following:

  • <150 µL: use 2 mL vials

  • <350 µL: use 5 mL vials