Run a sequence (LC/MS)

Before you run a sequence, you must develop an acquisition method and then develop a sequence.

Enter the information in the Single Sample Analysis window before running the method; once the run has started, this information cannot be changed. The information in this window is not part of the method, and it is not saved when the method is saved. When you are running a sequence, enter the information in the Sequence window.

You must open any method that was developed prior to OpenLab 2.4 at least once before you run a sequence.

Develop a method. See Method Editor for LC/MS Single Quadrupole.
Click Sequence.
Open a sequence.
In the Sequence window, enter the Properties information.
1. In the navigation pane at the left of the window, click General > Properties.
2. Select or clear Audit Trail Activated.
3. Enter the Description for the sequence.
Enter the Run Options information:
1. In the navigation pane at the left of the window, click General > Run Options.
2. Select or clear Use Barcode Reader Before Injection.
3. Click either Inject anyway or Abort current injection.
(optional) Add a column to the Sequence table.
1. In the navigation pane at the left of the window, click Injections > Table.
2. In the upper left corner of the Sequence table, click . The Choose Columns dialog opens.
3. Select the column (or columns) that you want to display.
4. Click Method Override Columns.
5. Select the column (or columns) that you want to display.
  - To override the SIM ions, see Rules to use to override SIM ions from a sequence (LC/MS).
  - To override fraction collection parameters, see Rules to override fraction collection parameters from a sequence (LC/MS)
6. Click OK.
Enter the Table information:
1. In the navigation pane at the left of the window, click Injections > Table.
2. Enter the information for each injection.
If needed, click Take. In this image, Take is already selected.
Click Run.

Run a sequence (LC/MS)

See Also