Chromatograms

What do you want to do?

• Learn more about chromatograms in the core Data Analysis software

• Use the 2D-LC functions

• Show cut annotations

• View details on cuts

• Select cuts

• Chromatogram Properties

With 2D-LC data, this window displays the signals of the first dimension. The time ranges where 2D signals have been acquired (the cuts ) are annotated.

The appearance of the individual cuts in the Chromatograms window depends on the cut type:

• Multiple Heart Cutting (MHC)

  In the Chromatograms window, each cut is highlighted with a border on the right side. There is no border on the left side. This illustrates that the flow goes continuously through the corresponding sample loop until the 2D-LC valve is switched.

  

  Figure Example of a MHC Chromatogram

• High-Resolution Sampling (HiRes) and Comprehensive 2D-LC

  In contrast to Multiple Heart Cutting, these cuts are shown with borders on both the left and the right side. This illustrates that the 2D-LC valve is switched to start the filling of the sample loop, and it is switched to stop it.

  

  Figure Example of a HiRes Chromatogram

NOTE

For comprehensive 2D-LC data acquired with the 2D-LC ChemStation Add-on, no information is available for the signals of the first dimension. No cut annotations are displayed in the Chromatograms window.