Align signals over two dimensions

In 2D-LC, signal alignment includes the following:

• Alignment of signals from multiple detectors in the same dimension; this ensures that peaks have the same retention time in all detectors.

• Shift of cut times in the second dimension; this ensures that you get correct cut chromatograms.

NOTE

Signal Alignment affects retention times. Therefore, we strongly recommend to first setup signal alignment in the processing method precisely, then reprocess your data.

This ensures that the shifts are applied in all windows, and you use the correct information, for example, to setup (timed) integration events, expected compound retention times, or any other time based actions. If you modify signal alignment settings after you have already set any retention times, review and adapt your retention time settings accordingly.