Calculate lag factors

Lag Factors are corrections that are applied to the mass axis due to ions taking a non-zero time to move through a quadrupole. This algorithm calculates and sets the mass assignment lag factors for the LC/MS

  1. Click Method.

    Ribbon_MethodLayout.jpg
  2. In the Acquisition Method window in the navigation pane, click Instrument Setup > SQ.

  3. In the navigation pane, select Tune > Manual Tune.

  4. In the navigation pane, select Tune > Manual Tune > Acquisition.

  5. If needed, click Take. In this image, Take is already selected.

    Ribbon_TakeControl.jpg
  6. (optional) Open a different tune file. Click TB_OpenATuneFile.jpg to open the Load Tune File dialog. Select a tune file and click Load.

  7. Click TB_TuneControl.jpg to lock the instrument for tuning. You need to unlock the instrument after you finish tuning.

  8. Select the Polarity. Select Positive or Negative.

  9. Click Calculate Log Factors.

  10. Click TB_UnlockTune.jpg to unlock the instrument. You need to unlock the instrument after you finish tuning.

See Also

Tune the MS manually

Tune > Acquisition Section