Ramp the lenses during manual tune

In this step, several parameters are ramped to find their optimum settings.

  1. In the navigation pane select Tune > Manual Tune

  2. In the navigation pane select Tune > Manual Tune > MS.

  3. If needed, click Take. In this image, Take is already selected.

    Ribbon_TakeControl.jpg
  4. If necessary, click TB_TuneControl.jpg to lock the instrument for tuning. You need to unlock the instrument after you finish tuning.

  5. Select the checkboxes next to the masses to ramp.

    Positive Polarity

    Electrospray

    APCI/MMI

    APPI

    AJS ESI

    Low mass

    118.09

    121.05

    121.05

    118.09

    Middle mass

    922.01

    922.01

    922.01

    922.01

    High mass for 6130C & 6135C

    2121.90

    2121.93

    2121.93

    2121.90

    High mass for 6120C & 6125C

    1521.97

    1521.97

    1521.97

    1521.97

    Negative Polarity

    Electrospray

    APCI/MMI

    APPI

    AJS ESI

    Low mass

    112.99

    119.04

    119.04

    112.99

    Middle mass

    1033.99

    805.99

    805.99

    1033.99

    High mass on 6130C & 6135C

    2233.90

    1805.92

    1805.92

    2233.90

    High mass on 6120C & 6125C

    1633.95

    1633.95

    1633.95

    1633.95

    Positive Polarity

  6. In the navigation pane under Tune > Manual Tune, select Optics.

  7. Select Lens1 DC for the Ramp Parameter.

  8. For the ramp parameters, enter -5 for From. Enter 5 for to. Enter 0.2 for the step.

  9. Click Ramp. The software will ramp lens 1 for the selected masses and produce a graph of voltage versus abundance for each mass.

  10. Click the point where abundance is maximized for the highest mass. You get better performance across the entire mass range. Click Apply.

  11. Select Iris for the Ramp Parameter.

  12. For the ramp parameters, enter -400 for From. Enter 400 for to. Enter 5 for the step.

  13. Click Ramp. The software will ramp the Iris for the selected masses and produce a graph of voltage versus abundance for each mass.

  14. Click the point where abundance is maximized for the highest mass. You get better performance across the entire mass range. Click Apply. Typical voltages are 350 for Positive polarity and 0 for Negative polarity.

See also

Manually tune the MS

Run Autotune

Tune the MS using Fast Scan

Tune > Manual Tune Section