Align signals manually

This function allows you to manually provide the delay times for detectors of both first and second dimension.

Preparations

• To be able to carry out the procedure as described, you need the privilege Processing Method > Edit signal parameters . Privileges are configured in the Control Panel.

• If the project enforces method approval, the method status must be Generic. For details, see Method approval.

• You know the transfer times for all detectors in each dimension.

  NOTE: Unlike in signal alignment for pure 1D Data Analysis, you need to know the transfer time for all detectors. The transfer for the first dimension is measured from the ¹D detector to the 2D-LC valve.

  See 2D-LC flow path.

1. Navigate to the General > Signals node of the processing method.

2. Select the Alignment tab.

3. Select Use manual delay.

4. Select the Use manual delay check boxes for the relevant detectors.

   If your method is not linked to any injections, the method lists all detectors from all loaded injections.

5. For each dimension where you want to align signals:

   • Enter the known transfer time under Manual delay (min).

   • Enter the retention times of a peak that is detected by all detectors.

   NOTE:
   In the first dimension, the first ¹D detector in the flow path has a longer transfer time than the second ¹D detector.
   In the second dimension, the first ²D detector has a shorter transfer time than the second ²D detector.
   See 2D-LC flow path.

In case of various Data Delay values, manual signal alignment is not recommended, as it would apply the same delay to different instrument configurations.

NOTE

Be careful if you reuse this method for injections with different detector configurations!