Signal

You can define the wavelengths of the excitation and emission, and specify signal acquisition.

Excitation

The excitation wavelength.

Limits: Zero Order (Ex) or 200 to 1200 nm in steps of 1 nm.

Emission

The emission wavelength.

Limits: Zero Order (Em) or 200 to 1200 nm in steps of 1 nm.

NOTE: The emission wavelength should be at least 10 nm greater than the excitation wavelength

Acquire Signal

Mark this check box to specify that the signal is stored in the CDS during data acquisition. When the check box is cleared, the signal is not stored.

Zero Order (Em)

Zero order sets the monochromator so that all light emitted from the sample will be reflected onto the detector.

Zero Order (Ex)

The full spectrum of light from the Xenon lamp illuminates the flow cell. Each compound can absorb its characteristic wavelength of light and then emit maximum fluorescence. An increased stray light level is inherent in this setting, and this will decrease sensitivity (signal-to-noise).