Integration is the final section. It is divided into two steps.
First, all EICs extracted in the previous step are integrated to determine peak areas. Any peak areas that are greater than or equal to 0.2% of total peak areas of EICs will be used to determine the MS purity.
Second, UV peaks are integrated. This is similar to the SST UV integration in that early and late eluting impurities are used to determine the integration of the UV main peak.
For each sample, extracted m/z values are shown in a table format. The graph shows overlaid signals for FLP and the selected ion's EIC, for standard and harsh conditions, in a relative scale. EICs have been integrated as per the processing method conditions.
If required, adjust integration manually in the EIC Integration window. Click and drag the integration points as needed. Click Integrate to apply changed settings.
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You cannot insert or delete peaks, nor perform other integration functions such as peak skimming. |
To facilitate and guide integration, each signal (FLP, standard EIC, harsh EIC) can be hidden by clearing its check box in the legend.
The UV Integration window shows the UV signal with recommended start and end times for the UV main peak.
OAA recommends the UV integration as follows, based on EICs of early and late eluting impurities:
To determine the UV main peak start time, the wizard selects the earliest eluting known impurity (from the m/z ion list).
Using the EIC for the earliest eluting impurity impurity (classified as known or unknown impurity for current sample), the software selects the integration time start, then subtracts the difference in retention times between FLP EIC and UV main peak.
The UV main peak is split using this retention time as the new peak start time.
To determine the UV main peak end time, the wizard selects the latest eluting known impurity (from the m/z ion list).
Using the EIC for the latest eluting impurity (classified as known or unknown impurity for current sample), the software selects the integration time end, then subtracts the difference in retention times between FLP EIC and UV main peak.
The UV main peak is split using this retention time as the new peak end time.
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To ensure proper integration, it is imperative that the UV main peak baseline has been established in the processing method. |
If necessary, adjust the UV main peak integration manually. Click and drag the dashed lines in the UV Integration window. When finished click Calculate to updated calculations.
If everything is correct, click Next to proceed. Confirm that you finished the integration settings. You will not be able to return to this step. Your changes are then saved as a new version of the result set.