Perform Negative Checktune

Perform a negative checktune prior to the oligo acquisition.

The requirements are specified by each laboratory. For example, consider the following parameters from the tune report:

  • m/z base peak should be within ± 0.1 amu of target mass in tune mix (standard tune mix includes 113, 602, 1034, 1634, 2234 m/z)

  • Source parameters in specification (voltage, gas temp/flow)

  • Gain settings

  • TIC height should meet requirement