UV purity is defined by the main peak (DAD signal). Any impurities or peak areas that elute before the main peak are considered early eluting impurities. Conversely, peak areas that elute after the main peak are late eluting impurities.
This calculation is performed by as follows:
where |
|
UVMain |
UV area of UV main peak |
UVimp |
UV area of early eluting and late eluting impurities |
UVTotal |
Summed UV area of all peaks |