With bracketed calibrations, the samples are bracketed by pre-sample and post-sample calibrations. The calibration standards between opening and closing brackets are processed first, and a calibration curve is calculated. This curve is then used to calculate the samples in this bracket.
For all bracketing modes except Custom, a Clear all calibration operation is automatically performed for all opening brackets. For the bracketing mode Custom, you can set the Run Type to Clear all calibration as required.
Bracketing is configured in the Injection List window. There are different bracketing modes:
Overall
The calibration curve is calculated with all calibration standards in the sequence, starting with the first one and finishing with the last one. All samples are reprocessed after the calibration curve has been calculated.
Non overlap
You must have at least three sets of standards in your sequence, and at least two standards in the middle. The standards in the middle of the sequence are each used in one single bracket only.
If there are more than two standards in the middle, they will be divided and allocated to the preceding and subsequent bracket. With uneven numbers of standards in the middle, the extra standard is allocated to the preceding bracket.
Overlap
You must have at least three sets of standards in your sequence. Standards in the middle of the sequence are used in two brackets (the preceding and the subsequent bracket).
Custom
Create brackets as required. In the Run type column, you can choose for each calibration standard individually which calibration levels shall be cleared. If you do not choose any run type, a bracket will be averaged with its predecessor.