Add a reference chromatogram

Preparations

• To be able to carry out the procedure as described, you need the privilege Processing Method > Edit signal parameters . Privileges are configured in the Control Panel.

• If the project enforces method approval, the method status must be Generic. For details, see Method approval.

1. Load the results that you want to use as a reference.

2. Open the method to which you want to add the reference chromatograms.

3. In the Processing Method window, select the General > Signals node.

4. Select the Reference Chromatogram tab.

5. If you have changed your data in the meantime, save all results.

   Only saved data can be added as a reference chromatogram.

6. Click .

   NOTE

   You can add up to five reference chromatograms.

7. In the Select reference chromatogram dialog, select the injection that you want to use as a reference. Click in the Signal field to choose the correct signal from the relevant injection.

   By default, the dialog shows the focused injection plus all pinned injections.

   The Signal drop-down list shows all signals for the selected injection that are supported for reference chromatograms and that are currently displayed in the Chromatograms window.

   MSD signals, 2D-LC signals, chromatograms created by data extraction, and instrument curves are not available as reference chromatograms.

   

   NOTE

   Each reference chromatogram is identified by the injection and signal that you choose in the Select reference chromatogram dialog. Its acquisition method name, path and version are also indicated as information. If a reference chromatogram already exists for a specific injection and signal, the existing reference chromatogram will be updated.

8. To select a signal from another loaded injection, clear the check box at the top of the dialog.

   The dialog then offers all loaded injections with all of their signals.

   MSD signals, 2D-LC signals, chromatograms created by data extraction, and instrument curves are not available as reference chromatograms.

   

9. Click OK.

10. Save the method, and link it to the relevant injections.

NOTE

If the audit trail is activated for your method, an entry about adding the reference chromatogram will be added to the method audit trail. The audit trail entry includes the version number of the result set that you used as a reference, as well as the name, path, and version of the acquisition method used to acquire the reference. For more information about versioning, see Versioning.