Step 2: Integration and Tuning

 

Example for Integration Optimizer with ChemStation integrator

1. Tune the integration parameters:

   • Use the sliders. For details on the slider parameters, see Tune using the sliders.

   • Tune the parameters graphically in the chromatogram. See Tune timed events in the chromatogram.

   • Tune a subsection

   • Add a custom baseline

2. To compare different combinations of integration parameters, create snapshots. See Compare integration parameters.

3. To view more details, adjust the displayed graph:

   • Zoom to baseline

   • Show or hide baseline annotations

   • Show or hide peak results

4. To compare the signal between different injections, pin the relevant injections. The injection that you selected in the first step is automatically pinned. For details, see Compare signals.

5. Evaluate how the parameters work with other data.

   When you select another injection, the relevant signal (for example, DAD1A) of this injection is automatically integrated using your current settings. The selected slider values and peak separation options remain unchanged. Only the value ranges are adjusted dynamically to the selected injection.

   The integration preview works only for the specific signal that you focused in step 1 of the Integration Optimizer (for example, DAD1A). If you select an injection that has no corresponding signal (in this example, DAD1A), your selection has no effect. The integration preview remains unchanged.

6. Click Next to proceed to the next step, or Previous to return to the previous step.

   Alternatively, to review the initial settings in a read-only mode, click Characterize Chromatogram in the upper navigation bar. Use the upper navigation bar then also to return to the current page.