Qualifier signals are used to confirm the identity of the target peak and verify that the response is not being influenced by matrix effects or co-eluting contaminants.
The peaks in the spectrum can be different fragments, adducts, or isotopic peaks from the same compound or from different compounds.
The most abundant peak in the MS spectrum is typically considered the target. The second to nth largest peaks are candidates for qualifiers. Extracted Ion Chromatograms (EICs) are generated for both target ion and qualifier ions.
The EICs or SIM signals of target and qualifier ions are compared. The qualifier will be listed as Passed or Failed in the injection results (see Evaluate MS qualifier results).
You set the number of qualifier ions, the acceptable retention time window and the required response percentage in the processing method ( Compounds > Identification node, Qualifier Setup tab).
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