Configure spectral scan and acquire scan data

1. Request tune control and select Manual Tune > Acquisition.
2. Select Scan from the Acquisition type menu.
3. Enter values for Low and High m/z to define the scan range.
4. Enter values for step size, speed, averages, and threshold.
5. Click Start acquiring data to start the scan.
6. Click Manual Tune > Parameters to change the value of a parameter and see real time results in the spectrum plot.
7. Click Stop acquiring data when you are satisfied with the results.
8. Click Save tune parameters with new file name and name this manual tune file.
9. Optionally, open a pdf of the manual tune file parameters.
10. Release tune control.