For each setpoint in the Analytical Gradient table that is marked in the Shift column, you can set up a gradient shift as a nested gradient. The gradient shift is used to align the 2D gradient composition with the 1D gradient composition. Select the line in the Analytical Gradient table for which to set up the shift gradient.
Time (min) |
Specify the time for the change of solvent composition in this field. The shifted gradient composition changes linearly from one setpoint to the next. |
B (%) |
Specify the percentage of solvent that is delivered by channel B at the specified time. Channel A always delivers the remaining volume, %A = (100-%B). The solvent composition changes linearly from one setpoint to the next. |
Appends a line to the gradient table. | |
Deletes the selected line in the gradient table | |
Clears the gradient table | |
Deletes the currently selected line in the gradient table and places it on the clipboard. | |
Copies the currently selected line in the gradient table to the clipboard. | |
Pastes the line on the clipboard into the gradient table according to its time. |