Calculate lag factors (LC/MS)

Lag Factors are corrections that are applied to the mass axis due to ions taking a non-zero time to move through a quadrupole. This algorithm calculates and sets the mass assignment lag factors for the LC/MS

1. Click Method.

2. In the Acquisition Method window in the navigation pane, click Instrument Setup > SQ.
3. In the navigation pane select Tune > Manual Tune.
4. In the navigation pane select Tune > Manual Tune > Acquisition
5. If needed, click Take. In this image, Take is already selected.

6. (optional) Open a different tune file. Click to open the Load Tune File dialog box. Select a tune file and click Load.
7. Click to lock the instrument for tuning. You need to unlock the instrument after you finish tuning.
8.  Click the Polarity. You can click Positive or Negative.
9. Click Calculate Lag Factors.
10. Click to unlock the instrument. You need to unlock the instrument after you finish tuning.

See Also

Tune the MS manually (LC/MS)

Tune > Acquisition Section