Tune > Manual Tune > Acquisition Section (LC/MS)

What do you want to do?

Manually tune the MS (LC/MS)

Prepare for manual tuning (LC/MS)

User Interface

Tune file

The name of the current tune file. You can use the icons in the Tune Toolbar to open and save a tune file.

Fast scan

Whether or not the current tune file is a Fast scan tune file.

Ion source

The source that is configured with the current instrument.

Diverter

Whether the stream from the LC is going into Waste or into the MS.

Calibrant on

Whether or not the calibrant is flowing.

Polarity

Positive or Negative.

Acquisition type

Scan, SIM, or Profile.

Acquisition Parameters

Dwell time (ms)

The time spent on each ion. See Dwell time (LC/MS).

Step size

The mass step size (in m/z) for profile scans. See Stepsize (LC/MS).

Samples

The number of data points collected at each mass during a scan. You specify the value for Scan and Profile. See Samples (LC/MS).

Averages

The number of profile scans to be averaged for each scan reported or displayed. You specify the value for Scan and Profile. See Averages (LC/MS).

Centroid

Whether or not to acquire Centroid data when acquiring data in Tune. See Data storage (LC/MS).

Threshold

The minimum value for a signal before it is reported. See Threshold (LC/MS)

Calculate Lag Factors button

Lag Factors are corrections that are applied to the mass axis due to ions taking a non-zero time to move through a quadrupole. When you click this button, it calculates and sets the mass assignment lag factors for the LC/MS.