Optimize 2D peak assignment by adjusting the method
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Optimize 2D peak assignment by adjusting the method

This procedure explains how to ensure that all relevant peaks in your cuts are assigned to the correct compound and contribute to the same ²D peak. The example used in the procedure shows a High Resolution sample that contains two compounds.

Preparations

  • To be able to carry out the procedure as described, you need the following privileges. Privileges are configured in the Control Panel.

    • Project Management  > Edit content of project

    • Processing Method  > Edit 2D peak assignment parameters

    • Data Processing  > Reprocess data

    • If the project enforces method approval, the method status must be Generic. For details, see Method approval.

  • You have loaded a single sample or result set with a 2D-LC method linked to it.

  1. To check the quality of the current 2D peak assignment, reprocess the data.

  2. Open the Contour Plot 2D window, and click (Show 2D peak regions) to see the assigned 2D peaks.

    In this example, the sample contains only two compounds, so there should be only two peak regions. However, the system found multiple peak regions.

  3. In the processing method, select 2D Peaks > Assignment .

  4. Adjust the values under Absolute ²D retention time window.

    In this example, the ²D retention times for one of the compounds vary between 0.900  and 0.910 .

    Therefore the Absolute ²D retention time window is set to 0.005 .

  5. Reprocess again, and check the result in the contour plot.

    The contour plot now shows only two expected peak regions.

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