Using the Fraction target mass Method Override parameters, you can override the compound name, monoisotopic mass, and formula for a fraction collection trigger signal in a method. The fraction collection peak trigger signal and all the instrument settings for the fraction collection trigger signal are preserved. You can override a single fraction target mass multiple times by specifying multiple compounds in the fraction target mass sequence column. See Rules to override fraction collection parameters from a sequence (LC/MS) for information on setting up a mass-based fraction collection method and using it with Fraction target mass Method Override fields.
Develop a method. See Method Editor for LC/MS Single Quadrupole.
Click Sequence.
Open a sequence.
If needed, click Take. In this image, Take is already selected.
In the Sequence window, enter the Properties and Run Options information. For more information, see Run a sequence (LC/MS).
Add the override column(s) to the Sequence table.
In the navigation pane at the left of the window, click Injections > Table.
In the upper left corner of the Sequence table, click . The Choose Columns dialog opens.
Select the Method Override Columns option.
Select the SQ: T1 Fraction target mass 1, SQ: T2 Fraction target mass 2, and/or SQ: T3 Fraction target mass 3 column.
If you add a Method Override column and enter values in the column, then even if the column is hidden, the Method Override will work. The only way to make the override not happen for that parameter is to clear the values in that column.
Any fraction trigger signals in the original method that are not overridden will be acquired as specified in the original method.
Click OK.
Enter the sequence information. For more information, see Run a sequence (LC/MS).
In the SQ: T1 Fraction target mass 1, SQ: T2 Fraction target mass 2, and/or SQ: T3 Fraction target mass 3 column, for each compound, enter the formula or monoisotopic mass and, optionally, the compound name. See Rules to override fraction collection parameters from a sequence (LC/MS). A formula or monoisotopic mass is required.
Fraction collection target mass overrides are recorded in the activity log.
Click Run.